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Short Communication | 28-June-2017

An Improve Protocol for PCR Using LM1 and LM2 Primers for Listeria monocytogenes Detection in Food Matrices

Several studies have observed that use of a conventional PCR protocol with primers LM1 and LM2 for the identification of the hlyA gene of Listeria monocytogenes generates non-specific PCR amplifications and false positives. For this reason, in this study we provide a modified PCR protocol that improves the specificity of the results obtained with LM1 and LM2 primers.

Angélica Godínez-Oviede, Gerardo M. Nava, Sofía M. Arvizu-Medrano, Montserrat Hernández-Iturriaga

Polish Journal of Microbiology, Volume 66 , ISSUE 2, 255–257

Original Paper | 04-September-2018

The Effect of Silver Nanoparticles on Listeria monocytogenes PCM2191 Peptidoglycan Metabolism and Cell Permeability

Listeria monocytogenes is Gram-positive bacterial pathogen, a causative agent of food poisoning and systemic disease – listeriosis. This species is still susceptible to several conventionally used antibiotics but an increase in its resistance has been reported. For this reason the search for new, alternative therapies is an urgent task. Silver nanoparticles seem to be the promising antibacterial agent. Minimal inhibitory concentration of silver nanoparticles was determined. Sublethal

KATARZYNA MARKOWSKA, ANNA MARIA GRUDNIAK, BARBARA MILCZAREK, KRYSTYNA IZABELLA WOLSKA

Polish Journal of Microbiology, Volume 67 , ISSUE 3, 315–320

Original Paper | 26-August-2016

Partial Characterization of Bacteriocin Produced by Halotolerant Pediococcus acidilactici Strain QC38 Isolated from Traditional Cotija Cheese

During a screening of lactic acid bacteria producing bacteriocin from Cotija cheese, the strain QC38 was isolated. Based on the 16S rRNA gene nucleotide sequencing (516 pb accession no KJ210322) and phylogenetic analysis, the isolate was identified as Pediococcus acidilactici. Neutralized cell-free supernatant was tested for antimicrobial activity against 17 Gram-negative and Gram-positive pathogens. Growth inhibition was achieved against Listeria monocytogenes (supplier or indication or source

Aurea I. Morales-Estrada, Ahidé López-Merino, Nestor Gutierrez-Mendez, Enrico A. Ruiz, Araceli Contreras-Rodríguez

Polish Journal of Microbiology, Volume 65 , ISSUE 3, 279–285

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