Serendipitous identification of Pratylenchus curvicauda from the grainbelt of Western Australia


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Journal of Nematology

Society of Nematologists

Subject: Life Sciences


ISSN: 0022-300X
eISSN: 2640-396X






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Serendipitous identification of Pratylenchus curvicauda from the grainbelt of Western Australia

Farhana Begum / John Fosu-Nyarko * / Shashi Sharma / Bill Macleod / Sarah Collins / Michael G. K. Jones

Keywords : Internal transcribed spacer (ITS), Molecular phylogeny, Morphology, Nematode classification, Pratylenchus spp, Pratylenchus curvicauda, Root Lesion Nematodes, Scanning Electron Microscopy (SEM), Taxonomy

Citation Information : Journal of Nematology. Volume 51, Pages 1-15, DOI:

License : (CC-BY-4.0)

Received Date : 17-February-2019 / Published Online: 29-July-2019



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  1. Al-Banna, L., Williamson, V. and Gardner, S. L. 1997. Phylogenetic analysis of nematodes of the genus Pratylenchus using nuclear 26S rDNA. Molecular Phylogenetics and Evolution 7(1):94–102.
  2. Carta, L. K., Handoo, Z. A., Skantar, A. M., Van Biljon, J. and Botha, M. 2002. Redescription of Pratylenchus teres Khan & Singh, 1974 (Nematoda: Pratylenchidae), with the description of a new subspecies from South Africa, and a phylogenetic analysis of related species. African Plant Protection 8(1 and 2):13–24.
  3. Castillo, P. and Vovlas, N. 2007. Pratylenchus (Nematoda: Pratylenchidae): diagnosis, biology, pathogenicity and management, Brill Leiden, Boston, MA.
  4. Cobb, N. A. 1917. A new parasitic nema found infesting cotton and potatoes. Journal of Agricultural Research 11:27–33.
  5. Collins, S., Wilkinson, C., Kelly, S., Hunter, H., Debrincat, L., Reeves, K. and Chen, K. 2017. The invisible threat: canola yield losses caused by root lesion nematode in WA. GRDC Updates, available at: wwwgiwaorgau/_literature_226079/Collins_Sarah_S17_The_invisible_threat (accessed December 15, 2018).
  6. Collins, S. J., Wilkinson, C. J., Kelly, S. J., Debrincat, L. and Hunter, H. F. 2015. Plant parasitic nematode causing strife in Western Australian broadacre crops. Australasian Plant Pathology Society Conference, Fremantle, September 14–16.
  7. De Luca, F., Reyes, A., Troccoli, A. and Castillo, P. 2011. Molecular variability and phylogenetic relationships among different species and populations of Pratylenchus (Nematoda: Pratylenchidae) as inferred from the analysis of the ITS rDNA. European Journal of Plant Pathology 130(3):415–26.
  8. De Luca, F., Fanelli, E., Di Vito, M., Reyes, A. and De Giorgi, C. 2004. Comparison of the sequences of the D3 expansion of the 26S ribosomal genes reveals different degrees of heterogeneity in different populations and species of Pratylenchus from the Mediterranean region, European Journal of Plant Pathology 111:949–57.
  9. Duncan, L. W., Inserra, R. N., Thomas, W. K., Dunn, D., Mustika, I., Frisse, L. M., Mendes, M. L., Morris, K. and Kaplan, D. T. 1999. Molecular and morphological analysis of isolates of Pratylenchus coffeae and closely related species, Nematropica 29(1):61–80.
  10. Filipjev, I. N. and Stekhoven, J. H. S. 1941. A manual of agricultural helminthology. E.J. Brill, Leiden.
  11. Fosu-Nyarko, J., Tan, J. C. H., Gill, R., Agrez, V. G., Rao, U. and Jones, M. G. K. 2015. De novo analysis of the transcriptome of Pratylenchus zeae to identify transcripts for proteins required for structural integrity, sensation, locomotion and parasitism. Molecular Plant Pathology 17(4):532–52.
  12. Godfrey, G. H. 1929. A destructive root disease of pineapple and other plants due to Tylenchus brachyurus. Phytopathology 19:611–29.
  13. Graham, T. W. 1951. Nematode root rot of tobacco and other plants, Bulletin 390, South Carolina Agricultural Experiment Station, Clemson Agricultural College, Clemson p. 25.
  14. Hodda, M. and Nobbs, J. 2008. A review of current knowledge on particular taxonomic features of the Australasian nematode fauna, with special emphasis on plant feeders, Australasian Plant Pathology 37(3):308–17.
  15. Hodda, M., Collins, S. J., Vanstone, V. A., Hartley, D., Wanjura, W. and Kehoe, M. 2014. Pratylenchus quasitereoides n. sp. from cereals in Western Australia. Zootaxa 3866(2):277–88.
  16. Huang, Y., Niu, B., Gao, Y., Fu, L. and Li, W. 2010. CD-HIT Suite: a web server for clustering and comparing biological sequences. Bioinformatics 26(5):680–2.
  17. Jones, J. T., Haegeman, A., Danchin, E. G. J., Gaur, H. S., Helder, J., Jones, M. G. K., Kikuchi, T., Manzanilla-López, R., Palomares-Rius, J. E. and Wesemael, W. M. L. 2013. Top 10 plant-parasitic nematodes in molecular plant pathology. Molecular Plant Pathology 14(9):946–61.
  18. Jones, M. G. K. and Fosu-Nyarko, J. 2014. Molecular biology of root lesion nematodes (Pratylenchus spp.) and their interaction with host plants. Annals of Applied Biology 164(2):163–81.
  19. Jones, M. G. K., Iqbal, S. and Fosu-Nyarko, J. 2016. Belowground defence strategies against migratory nematodes. Pp. 253–78 in Vos, C. M. F. and Kazan, K. (Eds), Belowground defence strategies in plants, Springer International Publishing Cham.
  20. Kearse, M., Moir, R., Wilson, A., Stones-Havas, S., Cheung, M., Sturrock, S., Buxton, S., Cooper, A., Markowitz, S. and Duran, C. 2012. Geneious basic: an integrated and extendable desktop software platform for the organization and analysis of sequence data. Bioinformatics 28(12):1647–9.
  21. Khan, E. and Singh, D. B. 1974. Five new species of Pratylenchus (Nematoda: Pratylenchidae) from India. Indian Journal of Nematology 4:199–211.
  22. Kumar, S., Stecher, G. and Tamura, K. 2016. MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Molecular Biology and Evolution 33:1870–4.
  23. Mckeand, J. B. 1998. Molecular diagnosis of parasitic nematodes. Parasitology 117:87–96.
  24. Mcwilliam, H., Li, W., Uludag, M., Squizzato, S., Park, Y. M., Buso, N., Cowley, A. P. and Lopez, R. 2013. Analysis tool web services from the EMBL-EBI, Nucleic Acids Research 41(W1):W597–W600.
  25. Mizukubo, T. and Minagawa, N. 1991. Morphometric differentiation of Pratylenchus neglectus (Rensch, 1924) and P. gotohi n. sp. (Nematoda: Pratylenchidae). Japanese Journal of Nematology 21:26–42.
  26. Nicol, P., Gill, R., Fosu-Nyarko, J. and Jones, M. G. K. 2012. De novo analysis and functional classification of the transcriptome of the root lesion nematode, Pratylenchus thornei, after 454 GS FLX sequencing. International Journal for Parasitology 42(3):225–37.
  27. Palomares-Rius, J. E., Castillo, P., Liébanas, G., Vovlas, N., Landa, B. B. and Navas-Cortés, J. A. 2010. Description of Pratylenchus hispaniensis n. sp. from Spain and considerations on the phylogenetic relationship among selected genera in the family Pratylenchidae. Nematology 12:429–51.
  28. Pourjam, E., Kheiri, A., Geraert, E. and Alizadeh, A. 1999. Variations in Iranian population of Pratylenchus neglectus and P. thornei. Iranian Journal of Plant Pathology 35:23–7.
  29. Rensch, D. 1924. Aphelenchus neglectus sp. N. Eine neue Nematodenart. Sonderabdruck aus dem Zoologischen Anzeiger 59:277–80.
  30. Ryss, A. 1988. World fauna of the root parasitic nematodes of the family Pratylenchidae (Tylenchida), USSR, Leningrad.
  31. Sher, S. A. and Allen, M. W. 1953. Revision of the genus Pratylenchus (Nematoda: Tylenchidae). University of California Publications in Zoology 57:441–7.
  32. Sherbakoff, C. D. and Stanley, W. W. 1943. The more important diseases and insect pests of crops in Tennessee. Tennessee Agriculture Experiment Station Bulletin 186:1–142.
  33. Siddiqi, M. R., Dabur, K. R. and Bajaj, H. K. 1991. Descriptions of three new species of Pratylenchus filipjev, 1936 (Nematoda: Pratylenchidae), Nematologia Mediterranea 19(1):1–7.
  34. Subbotin, S. A., Ragsdale, E. J., Mullens, T., Roberts, P. A., Mundo-Ocampo, M. and Baldwin, J. G. 2008. A phylogenetic framework for root lesion nematodes of the genus Pratylenchus (Nematoda): evidence from 18S and D2–D3 expansion segments of 28S ribosomal RNA genes and morphological characters. Molecular Phylogenetics and Evolution 48(2):491–505.
  35. Tamura, K., Nei, M. and Kumar, S. 2004. Prospects for inferring very large phylogenies by using the neighbor-joining method. Proceedings of the National Academy of Sciences (USA) 101:11030–35.
  36. Tamura, K., Stecher, G., Peterson, D., Filipski, A. and Kumar, S. 2013. MEGA6: molecular evolutionary genetics analysis version 6.0. Molecular Biology and Evolution 30(12):2725–29.
  37. Tan, J. C. H., Jones, M. G. K. and Fosu-Nyarko, J. 2013. Gene silencing in root lesion nematodes (Pratylenchus spp.) significantly reduces reproduction in a plant host. Experimental Parasitology 133(2):166–78.
  38. Taylor, S., Vanstone, V. A., Ware, A. H., Mckay, A. C., Szot, D. and Russ, M. H. 1999. Measuring yield loss in cereals caused by root lesion nematodes (Pratylenchus neglectus and P. thornei) with and without nematicide. Australian Journal of Agricultural Research 50(4):617–27.
  39. Vanstone, V. A., Hollaway, G. J. and Stirling, G. R. 2008. Managing nematode pests in the southern and western regions of the Australian cereal industry: continuing progress in a challenging environment. Australasian Plant Pathology 37(3):220–234.
  40. Waeyenberge, L., Viaene, N. and Moens, M. 2009. Species-specific duplex PCR for the detection of Pratylenchus penetrans. Nematology 11(6):847–57.
  41. Waeyenberge, L., Ryss, A., Moens, M., Pinochet, J. and Vrain, T. C. 2000. Molecular characterisation of 18 Pratylenchus species using rDNA restriction fragment length polymorphism. Nematology 2(2):135–42.
  42. Wang, H., Zhuo, K., Ye, W. and Liao, J. 2015. Morphological and molecular characterisation of Pratylenchus parazeae n. sp.(Nematoda: Pratylenchidae) parasitizing sugarcane in China. European Journal of Plant Pathology 143(1):173–91.
  43. Wood, W. B. 1988. The Nematode Caenorhabditis elegans, Cold Spring Harbour Laboratory, New York, NY.